Thermal hyperalgesia and cold allodynia induced after Pinch nerve injury. (A)Time course of thermal hyperalgesia tests after Pinch. The paw withdrawal latency (Seconds) after nociceptive hot stimulation significantly decreased at 30min time point in Pinch group (n=5) compared with sham group (n=5); This thermal hyperalgesia sustained for 7days. The contralateral paw has not shown this mechanical hyperalgesia compared with sham group animals (n=5). (B)cold allodynia tests after Pinch.Time course of cold stimulus scores measured by the Actone after Pinch-nerve injury (n=5). Ipsilateral hind paw displays a strongly but not significantly painful behavior measured by pain scores compared with sham group animals (n=5); This cold allodynia is not done in the contralateral paw compared with sham group animals (n=5). Data are expressed as mean±S.EM. *, P <0.05 compared with the sham group.