Influence of HGQ on the oxidative stress level in paraquat-treated N2 nematodes. Synchronized young adult nematodes were pretreated with or without HGQ at indicated concentrations at 20°C for 48 h prior to exposure to 10mM paraquat for 48 h. Approximately 1500 nematodes were collected and homogenized in PBST buffer. The relative level of ROS (A) and MDA content (B) were determined with DCF fluorescence Assay Kit and Lipid Peroxidation MDA Assay Kit. The results werw normalized by protein cotent. Data are shown as mean ± SEM of three independent experiments, and statistical significance is determined by one-way ANOVA followed by Tukey’s post hoc test. *p<0.05.