A and B, Two-nosed calf. C, Opening in the cranium. D, Brain, purulent meningitis (M), H&E, 500 μm. E, Higher magnification, purulent meningitis (arrowheads), H&E, 200 μm. F, Brain, vascular hyperemia (h) and inflammatory cell infiltration (arrow), H&E, 50 μm. G and H, Substansia grizea with meninges in the brain. H&E, 1000 μm. I, Synaptophysin positive areas in the region where the substantia alba in the area where substansia grizea is located in the inner brain region (arrows), IHC, 1000 μm. J, Synaptopysin positive areas in substansia grizea (arrowheads) and Synaptopysin negative areas in substansia alba, IHC, 500 μm. K, Brain, calcification (arrows), H&E, 50 μm. L, Brain, gliosis (arrowhead), H&E, 50 μm. M, Brain, neuronal degeneration (stars), H&E, 50 μm. N, Lung, hyperemia and bleeding (arrows), H&E, 200 μm. O, Liver, cystic structures (star), H&E, 500 μm. P, foci that resemble bacteria in terms of morphological structures (arrowhead) in cystic structures, H&E, 100 μm.