Expression of cathepsin D (CTSD) in the hypothalamus, pituitary, and ovary issues of different development stages. Wastern blotting was used to analyze CTSD expression in prepuberty, puberty, and postpuberty. (b, c, d) quantification of the CTSD expression level in different tissues and developmental stages, normalized to GAPDH expression Data are presentd as mean±standard deviation (SD). **P<0.01, nsP>0.05.