Alizarin Red and von Kossa staining of osteoblastic like-cells of first passage/ second and third week cultured in osteogenic medium with the scaffolds. (A-D) (Second week, orange red color was seen by positive cells in large amount in the vicinity of the scaffold surfaces and stained alizarin red stain at day 14. (E-H) Third week, orange red color was seen by positive cells in large amount interspersed with black mineral deposits with both (Alizarin Red/ von Kossa Staining) together at day 21. Inverted Phase Contrast Microscope Digital Camera Olympus, Magnification 10x.