comparation of decrease in viability, motility and plasm integrity of enzymatic and non-enzymatic treatment. NEZ= non-enzymatic treatment; EZ= enzymatic treatment; NEZ 5= control + ascorbic acid 5 mg/100 ml; NEZ 10 = control + ascorbic acid 10 mg/100 ml; NEZ 20 = control + ascorbic acid 20 mg/100 ml; EZ 25= control + catalase 25 mg/100 ml; EZ.