Effect of BHD on PC12 cell viability, (A) and PC12 cell apoptosis (B). Apoptosis was detected by flow cytometry (C). Results are shown as the mean ± SD (n = 3); ** P<0.01, vs. the control group; ## P< 0.01, vs. the HG group. Con, control group; HG, 100 mmol/L high glucose group; BHD-H, 800 µg/mL BHD group; BHD-L, 400 µg/mL BHD group; Met, 2.0 mmol/L metformin group.