TRAP stained multinucleated cells formed after 72h of osteoclast induction of RAW264.7 cells. The images in first row show the unstained RAW264.7 cultures after 72h of induction and CQ-H treatment at 4x magnification. The second row shows the cells stained for TRAP at 4x magnification. The pink colored cells indicate presence of TRAP positive osteoclasts. The third row shows TRAP stained cells at 10x magnification. The figures indicate that the positive control cultures have more stained multinucleated cells compared to CQ-H treated cells. The black arrows show the multinucleated cells. Images were taken from Nikon Eclipse TS-100 inverted microscope.