Expression of autophagy-related protein during adipogenesis differentiation. Subcutaneous pre-adipocyte in high-glucose medium (SH), subcutaneous pre-adipocyte in low-glucose medium (SL), visceral pre-adipocyte in high-glucose medium (VH), visceral pre-adipocyte in low-glucose medium (VL) at the indicated days (D0, D2, D4, D6, D8, D10). High-glucose medium supplemented 4500 mg/L glucose, low-glucose medium supplemented 1000 mg/L glucose. Protein band of LC3 and p62 in subcutaneous (A) and visceral pre-adipocyte (B). The cells extracts were prepared at the indicated days, and total cell proteins (25 µg) were separated on a 12% SDS-PAGE gel and analyzed by western blotting using abti-LC3 and anti-p62 antibody. Loading control was verified by GAPDH. Gray value quantification of LC-3 (C) and P62 (D). The values were expressed as mean ± SD, * p < 0.05, ** p < 0.01 comparisons were done between SH and VH, SL and VL, # p < 0.05, ## p < 0.01 comparisons were done between SH and SL, VH, and VL.