Alizarin Red and von Kossa staining of osteoblastic cells of first passage/ second and third week sub-cultured in osteogenic medium direct and indirect with the scaffolds by naked eye. (A-E) Direct contact at day 14, orange red color was seen by positive cells in large amount in the vicinity of the scaffold surfaces by alizarin red staining. (A-E) Indirect contact at day 21, brown -black mineral deposits (Mineralized areas) were seen by large cell colony in different fields of culture well with scaffolds extract by von Kossa staining.