VEGF mRNA expression was analyzed by quantitative real time polymerase chain reaction (qRT-PCR) on day 10 and 14 in avian growth plate of normal, thiram, FK228 treated avian chicks. Data expressed in arbitrary units as the means±SE (n= 6 for each group). Control group set to one thus equivalent to the N-fold difference. a, b letters designate significant difference (P <0.05).