Effect of RGFP966 and NSC74859 on the cell cycle of CT26+ colon cancer cells. (A) The representative expression of CDK4, Cyclin D1, CDK2, Cyclin E, p21 and p16 in CT26+ colon cancer cells and β-actin as the internal control for normalization. Quantitative analysis of CDK4 (B), Cyclin D1 (C), CDK2 (D), Cyclin E (E), p16 (F) and p21 (G) proteins. (H) Flow cytometric analysis of CT26+ colon cancer cells treated with RGFP966 and NSC74859. Each experiment was repeated three times, and all the data were presented as mean ± SEM in each case. One-way analysis of variance (ANOVA) was used when different data sets were compared. Statistically significant differences between different groups were indicated as *p < 0.05, **p < 0.01, ***p < 0.001and ns: no significance.