Effect of non-cytotoxic, non-detrimental concentrations of CQ-H (1, 10, and 100 ng/ml) on the differentiation of RAW264.7 cell line. The cells were cultured at 12000/cm2 density in 12-well culture plates. The control cells were fed with 0.05% DMSO supplemented medium. The positive control cells were fed with osteoclast induction medium (complete growth medium supplemented with 10ng/ml RANK-L and 0.05% DMSO). The CQ-H treated cells were fed with osteoclast induction medium containing 1, 10 and 100ng/ml CQ-H. After 48h of osteoclast induction, the cells fed with induction medium began to fuse with each other. After 72h of induction, mature, multinucleated cells were formed in the culture plate. The number of multinucleated cells was larger compared to CQ-H treated cultures. The black arrows show the large multinucleated cells. There were no large multinucleated cells in the control cultures. The images were taken at 4x magnification with Nikon Eclipse TS-100 inverted microscope.