Alignment of nucleotide sequence of product with Bos indicus bovine growth hormone (GH2) gene, GH2.1 allele (AY662651.1) using NCBI BLAST. 87% of the sequence aligned with three regions of Cattle. A, showing range 1 from position 31 to 58 aligned with first 28 nucleotides. B, showing range 2 from position 115 to 450 aligned from 85 to 420 nucleotides. C, showing range 3 from position 1 to 80 of gene aligned with position 422 to 478.Mutations are shown in turquoise color. Yellow shows the position of mutation in reference sequence.