Efficient production of LP-5 protein. Effect of temperature (A), IPTG concentration (B), and induction time on LP-5 production (C). SDS-PAGE analysis of LP-5 under optimized condition (D). Statistical analysis was performed using multiple t test via GraphPad Prism 6.0 (* p < 0.05, ** p < 0. 01, and p *** < 0.001).