Determination of trypanosomal protein expression depending on time of incubation in IPTG
Note: 1 – cell lysate; 2 – supernatant after centrifugation of cell lysate; 3 – pellet after centrifugation of cell lysate; 4 – pellet of cell lysate without IPTG; 5 – cell lysate pellet after 6 hours’ incubation with IPTG; 6 – pellet of cell lysate after 18 hours’ incubation with IPTG; mm – molecular weights marker.