Protein expression analysis by SDS-PAGE and western blotting.

A, Confirmation of HA protein expression resolved on SDS-PAGE gel stained with coomasie blue; Lane –ve, Rosetta culture as a –ve control; Lane 1-5, Total cell lysate with different treatments; Lane M, pre-stained protein marker (Fermentas); B, Confirmation of His-tagged HA protein on nitrocellulose membrane by western blotting; Lane M, pre-stained protein marker; Lane 1&2, Antigen-antibody reaction.