Electrophoresis results of PCR products on gel agarose 1% to identify the presence of the gene encoding M. hyopneumoniae P36 protein and the recombinant pGEM®-T Easy/P36 vector in E. coli TOP 10. Lane M: DNA size marker (100-1500 bp, Bio Basic), Lanes 1, 3, 5: PCR products with specific primer pairs for M. hyopneumoniae P36 gene; Lanes 2, 4, 6: PCR products with M13 primer pairs of the pGEM®-T Easy vector.