Differentiation multipotency characteristics of cartilage-derived CSPCs. (a) Intracellular lipid droplets of adipocytes accumulated in cytoplasm (bar, 50 μm) and were positive for Oil Red-O staining (bar, 20 μm); (b) Adipogenic gene expression of PPAR-γ and LPL was analyzed by RT-PCR. (c) Calcium deposits (bar, 50 μm) within induced osteoblasts were positive for Alizarin Red S staining (bar, 20 μm); (d) Osteogenic gene expression of COL1A2 and SPP1 was analyzed by RT-PCR.