Amino acid sequence analysis of the Youzhou dark goat KITL gene. a, homology of goat, sheep, bovine, rabbit, pig, mouse, and human KITL amino acid sequence. Sequence was aligned in DNAMAN. Amino acid sequence homology is shown by black shading (100%), pink shading (≥75%), and blue shading (≥50%). b, phylogenetic tree of KITL amino acid sequences. c, amino acid composition of goat KITL as determined in EXpASy. d, secondary structure prediction analysis of KITL conducted by Jpred. -: Random coil predicted by Jpred; H (red): α-helix; E (yellow): β-sheet. e, tertiary structure of the KITL protein in goat, sheep, bovine, rabbit, pig, mouse, and human.