Influence of different temperatures and pH values on the biosynthesis of α-amylase produced by BSAA-25 and BLAA-25. Influence of temperatures on the biosynthesis of α-amylase by (A) BSAA-25 and (B) BLAA-25 strains. Influence of pH values on the biosynthesis of α-amylase produced by (C) BSAA-25 and (D) BLAA-25 strains, using the medium M2 containing agro-waste (wheat bran). Each bar represents SEM for the mean of three replicates (p< 0.001).