Adipocyte differentiation from subcutaneous and visceral pre-adipocytes. HGM represents the differentiation of this row into high-glucose medium (supplemented 4500 mg/L glucose), and LGM represents the differentiation of this row into low-glucose medium (supplemented 1000 mg/L glucose). Subcutaneous (A) and visceral pre-adipocytes (B) were photographed under the bright-filed microscope at day 0 and day 10. Subcutaneous pre-adipocytes (C) and visceral pre-adipocytes (D) were stained with oil red-O dye was photographed under the bright-field microscope at day 0 and day 10. Cells at the indicated days were stained with Oil-Red O dyes as described in “Materials and methods”. (E) Histogram of triglyceride extraction after differentiation of subcutaneous in high-glucose medium (SH), subcutaneous in low-glucose medium (SL), visceral in high-glucose medium (VH), visceral in low-glucose medium (VL). The values were expressed as mean ± SD, * p < 0.05, ** p < 0.01, comparisons were done between SH and VH, SL and VL, # p < 0.05, ## p < 0.01, comparisons were done between SH and SL, VH, and VL.