The 0.1% SDS-15% polyacrylamide gel electrophoresis (SDS-PAGE) demonstrating the purified α-amylase. Lane 1, Novex™ Sharp Pre-stained Protein Standard; lane 2, cells carrying pET101-AMYL plasmid insoluble faction; lane 3, cells carrying pET101-AMYL plasmid soluble faction; lane 4, purified recombinant α-amylase.