The expression of PI3K, p-Akt, Akt, p-mTOR, mTOR, p-STAT3, STAT3 and HDAC3 proteins in CT26+ colon cancer mouse model with SD and KD treatment. (A) The representative expression of PI3K, p-Akt, Akt, p-mTOR, mTOR, p-STAT3, STAT3 and HDAC3 in CT26+ tumor tissues and β-actin as the internal control for normalization. Quantitative analysis of PI3K protein (B), the ratios of p-Akt to total Akt protein (C), the ratios of p-mTOR to total mTOR protein (D), HDAC3 protein (E) and the ratios of p-STAT3 to total STAT3 protein (F). Each experiment was repeated three times, and all the data were presented as mean ± SEM in each case. Independent-sample T-test was used when different sets of data were compared. Statistically significant differences between different groups were indicated as *p < 0.05, **p < 0.01 and ***p < 0.001.