Biofilm quantifications of S. Gallinarum strains (SG 119/Pak, SG 421/ India and SG 2a 189/China) using test tube and liquid interface coverslip assays. (a). Three strains were grown in test tubes over a time period of 3, 5 and 7 days. Each set was taken out after respectives time period and stained with 0.1% CV stain. OD580 [/] was measured. (b). Three strains were grown on coverslips submerged in petriplates having nutrient broth. After day 1 and day 2, each of the respective set of coverslips was processed for biofilm quantification as mentioned in test tubes assay using CV staining method. Experiment was run in triplicates. One way ANOVA followed by post hoc Tukey test was used to analyze results in SPSS (Version 13.0). Bas with no common superscript are significantly different (P<0.05).