Showed histological liver specimens were fixed in NBF (10%) for 6 hr. (A), 12 hr. (B), and 24 hr. (C). Fixed in Bouin’s for 6 hours (D), 12 hours (E), and 24 hours (F). Fixed in Carony,s for 6 hours (G) and 12 hours (H). In Zenker,s 12 hours (I) and 24 hours (J). In Clarke’s 6 hours (K) and 12 hours (L), staining of H and E 400X.