Fig. 1.
The tissue distribution of 3-1E gene in chicken in DNA microspheres vaccine group. 
A-E represent PCR amplification of the 3-1E gene in chicken at 7th, 14th, 21st, 28th, 35th day after primary inoculation, respectively. Lane M, DL1000 DNA Marker; Lanes 1-8, show products of the gastric, duodenal, splenic, lung, heart, liver, intestinal sample and blank control, respectively.