Agarose gel electrophoresis of PCR amplicon for gB gene of BoHV-1isolate separated on 1% agarose gel and stained with ethidium bromide (Positive PCR amplicons are at the size of approximately 478 bp). Lane 1 denotes for 100 bp DNA Ladder, Lane 2: Positive sample, Lane 3 negative control. Lane 4: positive control.