Schematic diagram of RKM1 (A) and RKM4 (B) regions of S. fimicola showing primers specific to SNP sites to perform multiplex PCR reaction. rkm1F1, rkm1F2, rkm1F3and rkm1F4 are specific for SNPs of NFS and SFS strains, while RKM1R1 is reverse primer specific for RKM1 region.