ALT promotes apoptosis in A2780 (parental) and A2780/CR (Cisplatin resistant) cells. (A) A2780 and A2780/CR cells were cultured and exposed to ALT for 24 h. Apoptosis rates were determined using flow cytometry. (B) A2780 and A2780/CR cells were treated with ALT as shown in figure. The cellular extracts were subjected to immunoblotting for the expression of caspase-3, cleaved PARP, γH2AX and B-actin. The numerical values below the immunoblots show the relative density of the band normalized to Beta actin from 3 repeated experiments.