Procedures for establishing pain-related mouse model and behavioral tests. Following the Pinch-nerve injury, mice in the Pinched group, and sham group were housed and subjected to thermal hyperalgesia behavior and cold allodynia tests consecutively within 7days and prolonged subsequently for 14days for Von-Frey tests. n = 5 per group. After Carrageenan injection (Carrageenan-treated group), and sham group were respectively injected with saline, mice were sacrificed after 15min , 90min, 1day and 4days and prolonged on the 7th day. After Dorsal Rhizotomy (DR) was completed, mice were housed for 7 days and subjected to DRGs dissection. n = 5 per group. The mice were performed Spared nerve injury (SNI) surgery; kept for 21days (3 weeks) and also subjected to DRGs dissection. n = 5 per group.