ELISA detection of protein contents and statistical analysis of colonic muscular layer in the four groups of rats after 4 days of intervention in vivo. A-C, On the 4th day of the imposed intervention, expressions of P-Ser396-Tau, P-Ser404-Tau and p-p38 were significantly reduced in the colonic muscular layers of rats in the OIC+SB203580 group compared with those in the OIC+NaCl and OIC+ANS groups (***P<0.001).