The effect of KD on the expression of Cyclins and CDKs in CT26+ colon cancer mouse model. (A) The representative expression of CDK4, Cyclin D1, CDK2, Cyclin E, p21 and p16 proteins in tumor tissues and β-actin as the internal control for normalization. Quantitative analysis of CDK4 (B) and Cyclin D1 (C), CDK2 (D) and Cyclin E (E), p16 (F) and p21 (G) proteins. Each experiment was repeated thrice, and all the data were presented as mean ± SEM in each case. Independent-sample T-test was used when different sets of data were compared. Statistically significant differences between different groups were indicated as *p < 0.05, **p < 0.01 and ***p < 0.001.