The expression and identification of SeFABP.

A, Schematic diagram of expression vector pET-30a(+) -SeFABP. B, The confirmation of the accuracy of vector. Lane 1: plasmid DNA. Lane 2: digested by ApaI and HindIII.  C, The expression of SeFABP identified by SDS-PAGE. Lane M, SDS-PAGE protein marker. Lane 0, control (no IPTG). Lane 1, induced at 0.5 mm IPTG at 37 °C for 16 h. Lane 2, supernatant after bacterial fragmentation. Lane 3, sediment after bacterial fragmentation