Analysis expression of q RT-PCR cell cycle releted genes (Cyclin E1, Cyclin D1, CDK6 and p21) treated HepG2 cells with LP-5 (A). Cell cycle anylasis of liver cancer cell lines HepG2 treated with LP-5  (0, 75, 100 and 125  g/ml and 48 h incubation time) (B). Detection of cell cycle related markers Cyclin-E1, Cyclin D1, CDK6 and P21 expressions in HepG2 liver cancer cells with LP-5 dose and time dependent mannaer  (0, 75, 100 and 125  g/ml and 48 h incubation time) (C). Statistical analysis was performed using multiple t test via GraphPad Prism 6.0 (* p < 0.05, ** p < 0. 01, and p *** < 0.001).