Effect of different concentrations of inhibitor UNC0642 administered for 48 h on relative expression of AMPKα2, ELL2 and Bcl-2 homology 3-only protein BIM genes determined by real-time PCR using HMBS as a reference gene for normalization and negative control (untreated) as calibrator. Compared with the respective controls, treatment with higher concentrations of UNC0642 upregulated the expression of AMPKα2 and ELL2 genes. Lower concentrations of inhibitor upregulated BIM expression in both cancerous and non-cancerous cell lines, whereas the higher concentrations downregulated the BIM gene expression in both cell lines. Statistical analysis was performed by using Two-way ANOVA with Tukey’s multiple comparison test.