ALKBH5 inhibits TGF-β1-induced ARPE-19 EMT.

(A), RT-qPCR disclosed ALKBH5 expression in TGF-β1 and control groups; (B), RT-qPCR showed the interference efficiency (sh-NC, sh-ALKBH5#1 and sh-ALKBH5#2) in untreated ARPE-19 cells and the overexpression efficiency (Vector and ALKBH5) in TGF-β1-treated ARPE-19 cells; (C), CCK-8 assay examined ARPE-19 cell viability after silencing or overexpression of ALKBH5; (D), Transwell assay uncovered ARPE-19 cell migration after depletion or overexpression of ALKBH5; (E), E-cadherin, ZO-1, N-cadherin and α-SMA expressions were examined by RT-qPCR after depletion or overexpression of ALKBH5.