15% SDS gel showing. A, fractions of denaturing immobilized metal affinity chromatography (IMAC); Lane 1: Pre-stained protein marker, Lane 2-9: 50- 500mM fractions. B, stages of purification of IL-2; Lane 1: Pre-stained Protein marker. Lane 2, Pellet; Lane 3, Supernatant; Lane 4, Solubilized inclusion bodies (IBs); Lane 5, IMAC purified IL-2; Lane 6, Renatured IL-2 after dialysis.