No interaction between HCMV pUL23 and pUL46 was detected in over-expressed Cos-7 cells. Cos-7 cell were transiently transfected with vectors expressing both Flag-tagged-pUL23, HA-tagged-pUL85 fusion proteins and Myc-tagged-pUL46 (co-transfected), lysed, and subjected to immunoprecipitation with anti-Flag antibody. The immunoprecipitated material was electrophoresed and detected with anti-Flag, anti-HA antibody and anti-Myc antibody, respectively