Fig. 1.

Experimental design to assess the NEDAP leg and neck logger tag variables for the estrus detection in dairy buffaloes. At day -35 transrectal ultrasonography (US) was done to determine the presence of CL on ovaries and verification of cyclicity at the start of experiment. First PGF2α injection was administered at the day -34 and then 11 days apart 2nd PGF2α injection at the day of -23 was administered and after PGF2α injection US was performed at 12-h intervals until ovulation was detected. The NEDAP leg and neck tag was attached at the day of -5 relation to day of estrus (day 0). From the day -3 to estrus onset (day 0) daily US was performed until ovulation and new CL was detected on ovaries. Insemination was done on the basis of AM: AM or PM: PM rule. Conception was determined at the day of +45 through ultrasonography.