The expression pattern toll-like receptors pathway genes in chicken erythrocytes on 0, 2, 6, and 10 h. This experimental study group was as follows: the control group (Con, erythrocytes treated with PBS, n = 3) and the experiment group (Exp, erythrocytes infected with M. synoviae, n = 3). Expression levels of TLR pathway genes were relatively calculated to that of 18S rRNA the housekeeping genes via qRT-PCR. SEM (standard error of mean) represented, by error bars. Bars with asterisks indicate a significant (*P < 0.05, **P < 0.01, ***P ≤ 0.001) Up or downregulated relative mRNA expression when compared to uninfected control group.