Confirmation of cloning of HA gene in pET30a expression vector and E. coli BL21 (D3).  A, Confirmation of successful digestion of TA vector containing HA gene and pET30a vector; B, Confirmation of HA gene-pET30a Ligation through restriction digestions; Lane 1-12, Restriction digestion of pET30a clones, Lane L, 1Kb DNA ladder; C, Confirmation of HA gene ligated in pET30a through PCR through PCR; Lane 1-3, Amplification of 1663bp of HA gene in pET30a; D, Confirmation of presence of HA gene ligated in pET30a transformed into Rosetta by PCR; Lane 1-7, Amplification of 1683bp HA gene ligated in pET30a plasmid; Lane 8, -ve control; Lane L, 1 Kb DNA Ladder.