Showing singleplex (A) and Multiplex-PCR amplification result (B) of six SNPs. A, Lane 1, 50 bp DNA ladder; lanes 2, 6, 10 and 14 IRF4, 3, 7, 11; Lane 15, SLC45A2; Lane 4, 8, 12 and 16 SLC24A4; Lanes 5, 9, 13 and 17 OCA2, 18, 19, 20; Lane 21 showed TYR and Lines 22, 23, 24 and 25, HERC2 SNP. In B, the 4% gel figure showed Line 1 50bp DNA marker; lines 2-5 showed the combined result of OCA2, TYR and IRF4, while lines number 6-9 showed the combined result of HERC2, SLC24A4 and SLC45A2.