ALKBH5 suppresses ZEB1 mRNA stability in an m6A-IGF2BP1-dependent manner. (A), RT-qPCR determined ZEB1 expression after depletion of ALKBH5 in untreated ARPE-19 cells and after ALKBH5 overexpression in TGF-β1-treated ARPE-19 cells; (B), Luciferase reporter assay unclosed the binding of ALKBH5 with ZEB1 promoter in ARPE-19 cells; (C), The stability of ZEB1 mRNA was determined by RT-qPCR after depletion or overexpression of ALKBH5 in ARPE-19 cells treated with α-amanitin; (D), MeRIP examined m6A modification of ZEB1 after depletion or overexpression of ALKBH5 in ARPE-19 cells; (E), RT-qPCR demonstrated the interference efficiency (sh-NC, sh-IGF2BP1#1 and sh-IGF2BP1#2) and the overexpression efficiency (Vector and IGF2BP1) in ARPE-19 cells; (F), RT-qPCR examined ZEB1 expression after depletion or overexpression of IGF2BP1 in ARPE-19 cells; (G), RIP assay validated the interaction between IGF2BP1 and ZEB1 in ARPE-19 cells; (H), RT-qPCR unclosed IGF2BP1 and ZEB1 expressions in ARPE-19 and TGF-β1-treated ARPE-19 cells under indicated transfections.