Alignment of nucleotide sequence of product with Bubalus bubalis growth hormone (GH) gene (KC107770.1) using NCBI BLAST. The sequence aligned with position 1 to 246 of Bubalus GH reference sequence. Mutations are shown in turquoise color. Yellow shows the position of mutation in reference sequence. TATA box is shown in orange and sequence of exon 1 is shown in blue.