Immunofluorescence analysis of the Gas6 in oocytes in vitro maturing. a and b, Identification of Gas6 in oocytes and COCs. The green staining indicates a FITC signal and the red stain indicates counterstaining with propidium iodide (PI). C, Relative fluorescence unit during the oocyte maturation was analyzed by image-pro plus 6.0 software. COCs, Cumulus-oocyte complexes. GV, germinal vesicle; GVBD, germinal vesicle broken down; MI, metaphase I; MII, metaphase II; COCs, cumulus-oocyte complexes; DEN, denuded oocytes. The results are presented as mean ± SEM.