Effect of different parameters on growth of E. coli (BL21 codon plus) cells transformed with pET28a (+)/IL-2. A, Effect of post induction time after induction with 0.5mM IPTG in different growth media. B, Effect of IPTG conc. in different growth media. C, Effect of time in different auto inducing media after induction with 5mM Lactose. D, Effect of lactose concentration in different auto-inducing media after induction with 5, 10, 15, 20 and 25mM Lactose concentrations, the curves represent growth patterns in different media, ♦ (LB), ■ (TB) and ▲M9.