Post-treatment histopathological examination of rabbit skin revealed varying effects depending on the treatment used. At 7 days, treatments with R and Lidocaine resulted in minimal changes, with mild degeneration of dermal collagen fibres and new hair follicle formation. Remifentanil treatments showed little inflammatory cell infiltration but no new hair follicles as A,B,C,D respectively. By 14 days, Remifentanil with gel showed moderate inflammation and collagen fibre depletion, while Lidocaine, Tramadol, and Remifentanil treatments showed minimal changes as in E,F, G,H respectively . At 21 days, Tramadol and Remifentanil treatments showed little inflammation and normal collagen fibers, while Lidocaine treatment showed no inflammatory reaction and normal mature dermal collagen fibers in I, J, K, L, M, respectively.