Morphology of muscle-derived stem cells at different stages of their isolation utilizing the preplate technique. MDSCs of first two passages is in homogenous, Afterwards, MDSCs were homogenous and the shape was round and spindle. A, slowly adhering cells are spherical after 1 h; B, slowly adhering cells are spherical after 2 h; C to K, cell morphologies at passage 1, passage 5, passage 10, passage 15, passage 20, passage 25, passage 30, passage 35 and passage 40 before passage (bar = 100μm). (l) Cell morphologies at passage 44 (bar = 100μm).