Expression of adipogenic transcription factors related protein during adipogenesis differentiation. Subcutaneous pre-adipocyte in high-glucose medium (supplemented 4500 mg/L glucose) (SH), subcutaneous pre-adipocyte in low-glucose medium (supplemented 1000 mg/L glucose) (SL), visceral pre-adipocyte in high-glucose medium (VH), visceral pre-adipocyte in low-glucose medium (VL) at the indicated days (D0, D2, D4, D6, D8, D10). High-glucose medium supplemented 4500 mg/L glucose, low-glucose medium supplemented 1000 mg/L glucose. Protein band of SREBP-1c and PPAR-γ in subcutaneous pre-adipocyte (A) and visceral pre-adipocyte (B). cells extracts were prepared at the indicated days, and total cell proteins (25 g) were separated on a 10% SDS-PAGE gel and analyzed by western blotting using abti-SREBP1c and anti-PPARγ antibody. Loading control was verified by GAPDH. Gray value quantification of SREBP-1c (C) and PPAR-γ (D). The values were expressed as mean ± SD, * p < 0.05, ** p < 0.01 comparisons were done between SH and VH, SL and VL, # p < 0.05, ## p < 0.01 comparisons were done between SH and SL, VH, and VL.